Description
Principle of the Assay: In an alkaline medium, Cu2+ was reduced to Cu+ by protein, and the purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with Cu+. This complex exhibits a strong absorbance at 562nm, and since the absorbancy strength is proportional to the protein concentration, determine the absorption value at 562nm, then compare with the standard curve, the protein concentration can be calculated.: